Coding
YebF-Cex

Part:BBa_K2922002:Design

Designed by: Junhong Chen   Group: iGEM19_XMU-China   (2019-10-13)


Exoglucanase fused at N-terminal with YebF secretion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 902
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 908
    Illegal NgoMIV site found at 1410
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 955
    Illegal SapI.rc site found at 1038


Design Notes

Overlap extension PCR from yebf gene and exoglucanase gene.


Source

YebF comes from Escherichia coli (Strain K12) and cex is from Cellulomonas fimi.

References