Coding
YebF-Cex
Part:BBa_K2922002:Design
Designed by: Junhong Chen Group: iGEM19_XMU-China (2019-10-13)
Exoglucanase fused at N-terminal with YebF secretion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 902
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 535
Illegal NgoMIV site found at 908
Illegal NgoMIV site found at 1410 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 955
Illegal SapI.rc site found at 1038
Design Notes
Overlap extension PCR from yebf gene and exoglucanase gene.
Source
YebF comes from Escherichia coli (Strain K12) and cex is from Cellulomonas fimi.